6T10

Crystal structure of YTHDC1 with fragment 28 (DHU_DC1_176)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.742, 103.524, 42.189
Unit cell angles	90.00, 105.73, 90.00

Refinement procedure

Resolution	40.610 - 1.480
R-factor	0.1909
Rwork	0.190
R-free	0.20990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.006
RMSD bond angle	0.899
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.700	1.570
High resolution limit [Å]	1.480	1.480
Number of reflections	53460	8464
<I/σ(I)>	13.72
Completeness [%]	98.2
Redundancy	3.43
CC(1/2)	0.999	0.707

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5