6T0D
Crystal structure of YTHDC1 with fragment 27 (DHU_DC1_256)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-24 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.975, 103.991, 42.026 |
Unit cell angles | 90.00, 105.39, 90.00 |
Refinement procedure
Resolution | 40.520 - 1.430 |
R-factor | 0.2043 |
Rwork | 0.203 |
R-free | 0.23280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4r3h |
RMSD bond length | 0.005 |
RMSD bond angle | 0.797 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.600 | 1.510 |
High resolution limit [Å] | 1.430 | 1.430 |
Number of reflections | 59103 | 9114 |
<I/σ(I)> | 9.3 | |
Completeness [%] | 96.4 | |
Redundancy | 3.52 | |
CC(1/2) | 0.997 | 0.592 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5 |