6T00

Crystal structure of Cold Shock Protein B (CSP-B) containing 4-F-Phe modified residues

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-07-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	54.940, 54.940, 57.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.790 - 2.100
R-factor	0.2013
Rwork	0.199
R-free	0.25170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1csp
RMSD bond length	0.010
RMSD bond angle	1.232
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.790	2.200
High resolution limit [Å]	2.100	2.100
Number of reflections	5551	692
<I/σ(I)>	17.23
Completeness [%]	99.9
Redundancy	12.43
CC(1/2)	0.999	0.429

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	100 mM CHES/ Sodium hydroxide pH 9.5, 1000 mM Sodium citrate tribasic