6SZN
Crystal structure of YTHDC1 with fragment 8 (DHU_DC1_006)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-03 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.830, 104.115, 42.115 |
| Unit cell angles | 90.00, 104.82, 90.00 |
Refinement procedure
| Resolution | 40.710 - 1.470 |
| R-factor | 0.1845 |
| Rwork | 0.183 |
| R-free | 0.21840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r3h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.739 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.710 | 1.550 |
| High resolution limit [Å] | 1.460 | 1.460 |
| Number of reflections | 54920 | 8115 |
| <I/σ(I)> | 10.73 | |
| Completeness [%] | 97.0 | |
| Redundancy | 3.37 | |
| CC(1/2) | 0.998 | 0.432 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5 |
