6SY4

TetR in complex with the TetR-binding RNA-aptamer K1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.2
Synchrotron site	BESSY
Beamline	14.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-01
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9184
Spacegroup name	P 42 21 2
Unit cell lengths	96.027, 96.027, 163.015
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.764 - 2.695
R-factor	0.2036
Rwork	0.201
R-free	0.24600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ns7
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	15.764	15.764	2.770
High resolution limit [Å]	2.695	12.050	2.700
Rmerge	0.196	0.026	3.697
Rmeas	0.200	0.026	3.772
Number of reflections	21786	297	1577
<I/σ(I)>	17.62	74.55	0.92
Completeness [%]	99.9	97.7	100
Redundancy	25.993	20.734	25.356
CC(1/2)	0.999	1.000	0.337

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	4% v/v Tacsimate pH 6.0, 12% w/v PEG 3350