6SX5

Full length Mutant Open-form Sodium Channel NavMs

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9688
Spacegroup name	I 4 2 2
Unit cell lengths	109.133, 109.133, 209.031
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.800 - 2.200
R-factor	0.222
Rwork	0.220
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hvx
RMSD bond length	0.010
RMSD bond angle	0.950
Data reduction software	xia2
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.800	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	32440	2774
<I/σ(I)>	14	4.5
Completeness [%]	100.0	100
Redundancy	19
CC(1/2)	0.996	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.5	277	0.05 M NaCl, 0.1 M MES buffer, pH 6.5, 37.8% v/v polyethylene glycol (PEG) 400 and 4% ethylene glycol