6SX5
Full length Mutant Open-form Sodium Channel NavMs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-04 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9688 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 109.133, 109.133, 209.031 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.800 - 2.200 |
R-factor | 0.222 |
Rwork | 0.220 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hvx |
RMSD bond length | 0.010 |
RMSD bond angle | 0.950 |
Data reduction software | xia2 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.800 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 32440 | 2774 |
<I/σ(I)> | 14 | 4.5 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19 | |
CC(1/2) | 0.996 | 0.969 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.5 | 277 | 0.05 M NaCl, 0.1 M MES buffer, pH 6.5, 37.8% v/v polyethylene glycol (PEG) 400 and 4% ethylene glycol |