6SWT

Affimer9 co-crystalised with the CH domains of alpha actinin 2.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9159
Spacegroup name	P 21 21 21
Unit cell lengths	46.192, 48.519, 146.993
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.500 - 1.200
R-factor	0.1499
Rwork	0.149
R-free	0.16400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a38 4n6t
RMSD bond length	0.008
RMSD bond angle	1.389
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.500	1.230
High resolution limit [Å]	1.200	1.200
Rmerge	0.073	0.802
Rmeas	0.079	0.948
Rpim	0.029	0.493
Number of reflections	103011	7078
<I/σ(I)>	10.4	1.6
Completeness [%]	98.9	93.3
Redundancy	5.9	3.4
CC(1/2)	0.997	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	20% PEG 3350, 0.2M ammonium tartrate