6SWK

The kinase domain of GanS, a histidine kinase from Geobacillus stearothermophilus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99
Spacegroup name	C 2 2 21
Unit cell lengths	48.950, 80.160, 116.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.777 - 1.742
R-factor	0.2208
Rwork	0.219
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6swj
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.850
High resolution limit [Å]	1.740	1.740
Rmerge	0.083	1.200
Number of reflections	23784	3574
<I/σ(I)>	20.7	1.7
Completeness [%]	99.1
Redundancy	23.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	30% PEG 3350, 1.05 M KSCN, 0.1 M Bis-Tris propane pH 6.3