6SWJ
The kinase domain of GanS, a histidine kinase from Geobacillus stearothermophilus (with Pt)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.99 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 48.830, 80.280, 116.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.719 - 1.762 |
| R-factor | 0.2017 |
| Rwork | 0.200 |
| R-free | 0.22980 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.870 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.062 | 1.186 |
| Number of reflections | 22974 | 6968 |
| <I/σ(I)> | 24.2 | 1.9 |
| Completeness [%] | 99.6 | |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 30% PEG 3350, 1.05 M KSCN, 0.1 M Bis-Tris propane pH 6.3 |
