6SVU
Reference structure of bovine trypsin (even frames of crystal x30)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P14 (MX2) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.8856 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.480, 56.760, 65.130 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.830 - 1.150 |
Rwork | 0.148 |
R-free | 0.20062 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 418G |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.830 | 42.830 | 1.180 |
High resolution limit [Å] | 1.150 | 5.160 | 1.150 |
Rmerge | 0.091 | 0.038 | 0.784 |
Rmeas | 0.108 | 0.045 | 1.043 |
Number of reflections | 126949 | 1481 | 3679 |
<I/σ(I)> | 7.42 | 24.91 | 0.69 |
Completeness [%] | 94.0 | 97.7 | 36.4 |
Redundancy | 3.34 | 3.428 | 1.509 |
CC(1/2) | 0.997 | 0.997 | 0.360 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | protein solution - 60 mg/ml trypsin, 6 mg/ml benzamidine and 3 mM CaCl2 in a 30 mM HEPES buffer pH 7.0. Well solution - 18% PEG 8000, 200 mM (NH4)2SO4, 50 mM HEPES pH 7, 3 mM CaCl2 and 6 mg/ml benzamidine. 1:1 ratio drops |