6SU5

Ph2119 endolysin from Thermus scotoductus MAT2119 bacteriophage Ph2119

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-17
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	31.999, 62.617, 74.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.980 - 1.200
R-factor	0.1462
Rwork	0.144
R-free	0.18890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	2.019
Data reduction software	XDS
Data scaling software	xia2
Phasing software	MrBUMP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.700	1.190
High resolution limit [Å]	1.170	1.170
Number of reflections	50883	2429
<I/σ(I)>	8.1	1.4
Completeness [%]	99.1
Redundancy	4.3
CC(1/2)	1.000	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		293	NaKHPO4, PEG 3350