6STW
Adenovirus 15 Fiber Knob protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-18 |
| Detector | DECTRIS EIGER2 X 500K |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.580, 89.180, 106.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.640 - 1.370 |
| R-factor | 0.1739 |
| Rwork | 0.173 |
| R-free | 0.19890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.722 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.640 | 39.640 | 1.410 |
| High resolution limit [Å] | 1.370 | 6.130 | 1.370 |
| Rmerge | 0.038 | 0.018 | 1.744 |
| Rmeas | 0.041 | 0.020 | 1.874 |
| Rpim | 0.015 | 0.007 | 0.676 |
| Total number of observations | 881087 | 10358 | 66057 |
| Number of reflections | 119296 | 1495 | 8721 |
| <I/σ(I)> | 18.4 | 56.7 | 1.1 |
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 7.4 | 6.9 | 7.6 |
| CC(1/2) | 1.000 | 0.999 | 0.478 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 294 | 0.1M SPG, 25% w/v PEG 1500 |
