6STV

Adenovirus 29 Fiber Knob protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-18
Detector	DECTRIS EIGER2 X 500K
Wavelength(s)	0.95372
Spacegroup name	P 2 3
Unit cell lengths	104.290, 104.290, 104.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.850 - 1.600
R-factor	0.178
Rwork	0.177
R-free	0.20060
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.699
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	73.850	73.740	1.630
High resolution limit [Å]	1.600	8.760	1.600
Rmerge	0.107	0.024	3.603
Rmeas	0.109	0.025	3.686
Rpim	0.023	0.006	0.776
Total number of observations		7141	54939
Number of reflections	50042	355	2447
<I/σ(I)>	18.1	98.2	1
Completeness [%]	100.0	99.9	100
Redundancy	22.6	20.1	22.5
CC(1/2)	1.000	1.000	0.444

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	294	0.1M SPG, 25% w/v PEG 1500