6STU
Adenovirus 30 Fiber Knob protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-18 |
| Detector | DECTRIS EIGER2 X 500K |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 63.350, 87.360, 217.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.760 - 2.390 |
| R-factor | 0.2194 |
| Rwork | 0.217 |
| R-free | 0.26810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.722 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 54.760 | 54.760 | 2.450 |
| High resolution limit [Å] | 2.390 | 10.690 | 2.390 |
| Rmerge | 0.125 | 0.033 | 1.484 |
| Rmeas | 0.134 | 0.036 | 1.588 |
| Rpim | 0.049 | 0.014 | 0.561 |
| Total number of observations | 365841 | 4064 | 28110 |
| Number of reflections | 48896 | 642 | 3589 |
| <I/σ(I)> | 11.2 | 39.7 | 1.4 |
| Completeness [%] | 100.0 | 99.1 | 100 |
| Redundancy | 7.5 | 6.3 | 7.8 |
| CC(1/2) | 0.998 | 0.997 | 0.623 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 0.1M SPG, 25% w/v PEG 1500 |
