6STT
Adenovirus 29 Fiber Knob protein in complex with Sialic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-05-18 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 2 3 |
| Unit cell lengths | 104.236, 104.236, 104.236 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.250 - 1.540 |
| R-factor | 0.1423 |
| Rwork | 0.140 |
| R-free | 0.18780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 60.250 | 60.180 | 1.570 |
| High resolution limit [Å] | 1.540 | 8.440 | 1.540 |
| Rmerge | 0.093 | 0.030 | 2.370 |
| Rmeas | 0.096 | 0.031 | 2.425 |
| Rpim | 0.020 | 0.007 | 0.512 |
| Total number of observations | 7378 | 62276 | |
| Number of reflections | 55982 | 394 | 2787 |
| <I/σ(I)> | 17.9 | 77 | 1.4 |
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 22.4 | 18.7 | 22.3 |
| CC(1/2) | 1.000 | 1.000 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 0.1 M SPG Buffer, 25% PEG 1500 |
