6STC

Crystal structure of the tick chemokine-binding protein Evasin-4 (SG 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-29
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	P 32 2 1
Unit cell lengths	71.066, 71.066, 43.059
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.790 - 1.690
R-factor	0.1769
Rwork	0.176
R-free	0.19890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6st4
RMSD bond length	0.014
RMSD bond angle	1.724
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.060	1.720
High resolution limit [Å]	1.690	1.690
Rmerge	0.054	0.928
Number of reflections	14083	722
<I/σ(I)>	11.7	1.4
Completeness [%]	98.3
Redundancy	3.8
CC(1/2)	0.999	0.496

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	40% PEG 400, 0.1 M NaAc pH 4.5