6STB

Crystal structure of the strawberry pathogenesis-related 10 (PR-10) Fra a 1.02 protein, Q64W mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.966
Spacegroup name	P 21 21 21
Unit cell lengths	41.836, 59.516, 131.218
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.080 - 2.270
R-factor	0.205
Rwork	0.202
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5amw
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.201	2.531
High resolution limit [Å]	2.267	2.267
Rmerge	0.126	1.241
Rmeas	0.140
Rpim	0.060	0.504
Number of reflections	10544	528
<I/σ(I)>	8.4	1.4
Completeness [%]	90.5
Redundancy	5
CC(1/2)	0.997	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Bis-Tris pH5.5 200 mM Magnesium chloride 26.5% PEG3350