6ST9
Crystal structure of the strawberry pathogenesis-related 10 (PR-10) Fra a 1.02 protein, D48R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-11 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 105.584, 41.831, 91.793 |
| Unit cell angles | 90.00, 116.15, 90.00 |
Refinement procedure
| Resolution | 82.400 - 1.970 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5amw |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.400 | 2.139 |
| High resolution limit [Å] | 1.970 | 1.970 |
| Rmerge | 0.063 | 1.209 |
| Rmeas | 0.071 | |
| Rpim | 0.032 | 0.569 |
| Number of reflections | 19595 | 980 |
| <I/σ(I)> | 12.6 | 1.2 |
| Completeness [%] | 91.6 | 59.5 |
| Redundancy | 4.7 | 5.4 |
| CC(1/2) | 0.998 | 0.451 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM Bis-Tris pH5.5 200 mM Ammonium Sulfate 34% PEG3350 |
