6ST4
Crystal structure of the tick chemokine-binding protein Evasin-4 (SG 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9772 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.999, 67.999, 41.575 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.890 - 1.290 |
| R-factor | 0.179 |
| Rwork | 0.178 |
| R-free | 0.19430 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.820 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.890 | 1.320 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.143 | 0.151 |
| Number of reflections | 28221 | 2050 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 100.0 | |
| Redundancy | 9.6 | |
| CC(1/2) | 0.990 | 0.552 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 40% ethylene glycol, 0.2 M (NH4)2SO4, 0.1 M Phosphate-citrate pH 4.2 |
