6SSC
N-acetylmuramoyl-L-alanine amidase LysC from Clostridium intestinale URNW
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97951 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.400, 53.780, 76.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 14.900 - 1.210 |
R-factor | 0.1225 |
Rwork | 0.120 |
R-free | 0.16070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6su5 |
RMSD bond length | 0.018 |
RMSD bond angle | 2.222 |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.900 | 1.230 |
High resolution limit [Å] | 1.210 | 1.210 |
Rmerge | 0.070 | 1.110 |
Number of reflections | 47839 | 2324 |
<I/σ(I)> | 12.1 | 1.3 |
Completeness [%] | 99.8 | |
Redundancy | 5.3 | |
CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 7.4 | 293 | NaKHPO4, PEG 3350 |