6SS5
Structure of the arginase-2-inhibitory human antigen-binding fragment Fab C0020187
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-12-02 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.91587 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.320, 103.370, 164.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.354 - 1.780 |
| Rwork | 0.178 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6srv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.781 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.355 | 1.820 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.160 | 4.110 |
| Number of reflections | 58306 | 3268 |
| <I/σ(I)> | 8.1 | 0.5 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 10.4 | 8.5 |
| CC(1/2) | 1.000 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 100 mM HEPES pH 7.5 70% MPD |
