6SS0
Structure of the arginase-2-inhibitory human antigen-binding fragment Fab C0021181
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.400, 67.370, 94.330 |
| Unit cell angles | 90.00, 100.24, 90.00 |
Refinement procedure
| Resolution | 46.544 - 1.700 |
| Rwork | 0.189 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6srv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.313 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.548 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.150 | 2.590 |
| Number of reflections | 88773 | 4541 |
| <I/σ(I)> | 5.9 | 0.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.4 | 5.4 |
| CC(1/2) | 1.000 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 100 mM SPG pH 7.0 25% PEG1500 PACT A4 SPG: succinic acid, phosphate, glycine |
