6SRX
Structure of the arginase-2-inhibitory human antigen-binding fragment Fab C0021158
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-11 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97242 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.732, 122.670, 63.985 |
| Unit cell angles | 90.00, 114.89, 90.00 |
Refinement procedure
| Resolution | 48.955 - 1.900 |
| Rwork | 0.234 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6srv |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.341 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.960 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.110 | 1.450 |
| Number of reflections | 67778 | 4374 |
| <I/σ(I)> | 6.4 | 0.7 |
| Completeness [%] | 98.3 | 98.5 |
| Redundancy | 3.4 | 3.5 |
| CC(1/2) | 1.000 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 291 | 100 mM NaAcO pH 5.0 20% PEG6000 200 mM LiCl |
