6SRT
Endolysine N-acetylmuramoyl-L-alanine amidase LysCS from Clostridium intestinale URNW
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-19 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.340, 53.980, 77.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.200 - 1.210 |
| R-factor | 0.1213 |
| Rwork | 0.119 |
| R-free | 0.15740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6su5 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.281 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 1.230 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.078 | 1.220 |
| Number of reflections | 48199 | 2370 |
| <I/σ(I)> | 9.3 | 1.1 |
| Completeness [%] | 99.9 | |
| Redundancy | 5.2 | |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.4 | 293 | NaKHPO4, PEG 3350 |
