6SRN
Structural basis for control of antibiotic production by bacterial hormones
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 67.210, 67.210, 92.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.340 - 1.500 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.20710 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.862 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.340 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 34705 | 1507 |
| <I/σ(I)> | 17.9 | |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 11.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 10-15% PEG 3350 and 0.2-0.25 M magnesium formate |
