6SQK

Crystal structure of mouse PRMT6 with modified H7-4 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976251
Spacegroup name	P 21 21 2
Unit cell lengths	118.632, 142.829, 41.701
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.629 - 1.400
R-factor	0.1911
Rwork	0.190
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4c03
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3546)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.610	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.134	2.523
Rmeas	0.140	2.629
Rpim	0.039	0.734
Number of reflections	140415	13708
<I/σ(I)>	9.8	0.9
Completeness [%]	100.0	98.9
Redundancy	12.7	12.7
CC(1/2)	0.998	0.395

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	PEG 3,350 20%, Mg(CH3COO)2 200 mM, HEPES-NaOH pH 7.0 100 mM