6SQI
Crystal structure of mouse PRMT6 with C-terminal TEV cleavage site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-15 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.54178 |
| Spacegroup name | I 41 |
| Unit cell lengths | 79.644, 79.644, 117.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.690 - 1.600 |
| R-factor | 0.1474 |
| Rwork | 0.144 |
| R-free | 0.16730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4c03 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13rc2_2975) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.690 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.049 | 0.736 |
| Rmeas | 0.052 | 0.913 |
| Rpim | 0.016 | 0.529 |
| Number of reflections | 47624 | 2048 |
| <I/σ(I)> | 22.1 | 1.4 |
| Completeness [%] | 99.0 | 85.2 |
| Redundancy | 8.6 | 2.4 |
| CC(1/2) | 1.000 | 0.436 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG Smear High 8%, NaCHOO 40 mM, CaCl2 40 mM, PIPES pH 6.8 100 mM |
