6SQ4
Crystal structure of mouse PRMT6 in complex with inhibitor U2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-06 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.950, 118.101, 72.093 |
| Unit cell angles | 90.00, 103.45, 90.00 |
Refinement procedure
| Resolution | 35.058 - 1.700 |
| R-factor | 0.1828 |
| Rwork | 0.181 |
| R-free | 0.21110 |
| Structure solution method | SAD |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | AutoSol |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.170 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.053 | 0.934 |
| Rmeas | 0.058 | 1.125 |
| Rpim | 0.023 | 0.621 |
| Total number of observations | 361459 | |
| Number of reflections | 74687 | 3909 |
| <I/σ(I)> | 16.5 | 1.2 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 4.8 | 3 |
| CC(1/2) | 0.999 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 298 | PEG 3,350 20%, NaI 200 mM, Tris-HCl pH 7.5 100 mM |
