6SPN
Structure of the Escherichia coli methionyl-tRNA synthetase complexed with beta-methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.225, 45.230, 86.252 |
| Unit cell angles | 90.00, 107.36, 90.00 |
Refinement procedure
| Resolution | 41.687 - 1.450 |
| R-factor | 0.947 |
| Rwork | 0.093 |
| R-free | 0.12580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.700 | 1.540 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Number of reflections | 101094 | 15649 |
| <I/σ(I)> | 20.9 | 7.9 |
| Completeness [%] | 98.4 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.999 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 281 | 1.08 M ammonium citrate, 10 mM potassium phosphate, pH 7.0 |
