6SOZ
Glycosylated Trypanosoma brucei transferrin receptor in complex with human transferrin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.180, 117.870, 134.550 |
| Unit cell angles | 90.00, 111.45, 90.00 |
Refinement procedure
| Resolution | 39.590 - 3.420 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.24100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.600 | 3.480 |
| High resolution limit [Å] | 3.420 | 3.420 |
| Number of reflections | 25075 | 1291 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.988 | 0.692 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12% (w/v) PEG 5000 MME, 12% 2-methyl-2,4-pentanediol, 0.1 M MES, pH 6.5 |
