6SNZ
Crystal structure of lamin A coil1b tetramer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07169 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 88.070, 37.470, 101.680 |
| Unit cell angles | 90.00, 94.12, 90.00 |
Refinement procedure
| Resolution | 35.150 - 2.600 |
| Rwork | 0.254 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CCFold generated model |
| Data reduction software | XDS |
| Data scaling software | autoPROC |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.150 | 2.693 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.132 | 1.645 |
| Rmeas | 0.145 | |
| Rpim | 0.058 | 0.914 |
| Number of reflections | 20594 | 1810 |
| <I/σ(I)> | 8.62 | |
| Completeness [%] | 97.0 | 86.65 |
| Redundancy | 6 | 4.1 |
| CC(1/2) | 0.999 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | PEG 8K 10%, Imodazole 0.1M, Calcium acetate 0.2M |
