6SNO
Crystal structures of human PGM1 isoform 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-03 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.954, 53.351, 76.321 |
| Unit cell angles | 90.00, 98.44, 90.00 |
Refinement procedure
| Resolution | 43.570 - 2.700 |
| R-factor | 0.1996 |
| Rwork | 0.197 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.564 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.570 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.109 | 0.781 |
| Rmeas | 0.122 | 0.872 |
| Rpim | 0.053 | 0.380 |
| Number of reflections | 16153 | 2142 |
| <I/σ(I)> | 10.6 | 1.9 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 4.9 | 4.9 |
| CC(1/2) | 0.996 | 0.799 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | KCl, MgCl2, Na cacodylate pH 6.5 and PEG4000 |
