6SNA

Crystal structure of Antirestriction ArdC protein from R388 plasmid. Mn(II)-bound structure.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 32
Unit cell lengths	116.499, 116.499, 162.123
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.820 - 2.700
R-factor	0.2207
Rwork	0.222
R-free	0.29430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6i89
Data reduction software	MOSFLM (7.2.2)
Data scaling software	SCALA (3.3.21)
Phasing software	MOLREP (CCP4 7.0.076)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.820	2.850
High resolution limit [Å]	2.700	2.700
Number of reflections	66345	6492
<I/σ(I)>	25.69
Completeness [%]	96.6
Redundancy	17.5
CC(1/2)	0.997	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	25 % v/v ethylene glycol