6SM1

Wild type immunoglobulin light chain (WT-1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-31
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97625
Spacegroup name	P 63 2 2
Unit cell lengths	104.500, 104.500, 55.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 1.550
R-factor	0.143
Rwork	0.142
R-free	0.16490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nki
RMSD bond length	0.007
RMSD bond angle	1.315
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.650
High resolution limit [Å]	1.550	1.550
Rmerge	0.046	0.587
Number of reflections	26220	4387
<I/σ(I)>	20.9	3.2
Completeness [%]	99.7	99.8
Redundancy	6.2
CC(1/2)	0.999	0.868

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1M HEPES, 0.2 M CaCl2, 28% PEG 400