6SM1
Wild type immunoglobulin light chain (WT-1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-31 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 104.500, 104.500, 55.220 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 1.550 |
| R-factor | 0.143 |
| Rwork | 0.142 |
| R-free | 0.16490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4nki |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.650 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.046 | 0.587 |
| Number of reflections | 26220 | 4387 |
| <I/σ(I)> | 20.9 | 3.2 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 6.2 | |
| CC(1/2) | 0.999 | 0.868 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES, 0.2 M CaCl2, 28% PEG 400 |
