6SLN

Structure of the RagAB peptide transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.917
Spacegroup name	P 21 21 21
Unit cell lengths	129.781, 142.734, 250.048
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	123.960 - 2.610
R-factor	0.2096
Rwork	0.207
R-free	0.26420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sli
RMSD bond length	0.008
RMSD bond angle	1.025
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	142.700	2.650
High resolution limit [Å]	2.610	2.610
Rmeas	0.278	2.079
Rpim	0.102	0.752
Number of reflections	141223	6762
<I/σ(I)>	5.6	1
Completeness [%]	99.9
Redundancy	7.3
CC(1/2)	0.995	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	293	15% PEG1000 0.05 M Li-sulphate 0.05 M Na-phosphate monobasic 0.08 M Na-citrate