6SLK
Diaminobutyrate acetyltransferase EctA from Paenibacillus lautus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 176.007, 176.007, 61.781 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 124.460 - 2.200 |
| R-factor | 0.1705 |
| Rwork | 0.170 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.983 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 124.500 | 2.070 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 70540 | 10980 |
| <I/σ(I)> | 10.65 | 1.08 |
| Completeness [%] | 99.2 | 95.5 |
| Redundancy | 12.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 285 | 0.2 M lithium sulphate, 0.2 M sodium acetate, 0.1 M Hepes pH 7.5, 25 % (w/v) PEG 4000 |
