6SLJ

Structure of the RagAB peptide transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-15
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	130.597, 142.021, 241.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.010 - 3.040
R-factor	0.2254
Rwork	0.222
R-free	0.29520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sli
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.530	3.090
High resolution limit [Å]	3.040	3.040
Rmeas	0.430	1.551
Rpim	0.157	0.558
Number of reflections	87154	4279
<I/σ(I)>	3.7	1.3
Completeness [%]	100.0
Redundancy	7.4
CC(1/2)	0.950	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.5	293	22% PEG400 0.07 M NaCl 0.05 M Na-citrate