6SLD

Structure of the Phosphatidylcholine Binding Mutant of Yeast Sec14 Homolog Sfh1 (S175I,T177I) in Complex with Phosphatidylinositol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000050
Spacegroup name	P 21 21 21
Unit cell lengths	48.220, 70.810, 90.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.095 - 1.400
R-factor	0.1445
Rwork	0.143
R-free	0.16350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.039
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.095	45.095	1.430
High resolution limit [Å]	1.396	6.240	1.400
Rmerge	0.092	0.035	1.274
Rmeas	0.095	0.037	1.331
Number of reflections	61867	791	4321
<I/σ(I)>	17.47	54.64	1.92
Completeness [%]	99.7	99.9	95.6
Redundancy	12.746	12.148	11.732
CC(1/2)	0.999	0.999	0.728

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.8	277	40% (w/v) PEG3350, 10% Glycerol (w/v), 100mM Kaliumphosphate 100mM Ammoniumacetat