6SL3
ALPHA-ACTININ FROM ENTAMOEBA HISTOLYTICA in orthorhombic space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-08-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.005755 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 43.210, 71.830, 241.680 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.230 - 3.100 |
| R-factor | 0.26829 |
| Rwork | 0.259 |
| R-free | 0.32451 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sl2 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.240 | 3.170 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.133 | |
| Number of reflections | 14460 | 967 |
| <I/σ(I)> | 8.57 | 0.48 |
| Completeness [%] | 99.1 | |
| Redundancy | 7 | |
| CC(1/2) | 0.990 | 0.433 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 92 mM bicine/trizma base (pH 8.5), 27.6 mM CaCl2, 27.6 mM MgCl2, 11.5% (w/v) PEG 3,350, 11.5% (w/v) PEG 1,000, 11.5% (v/v) MPD |
