6SK4

Methyltransferase MtgA from Desulfitobacterium hafniense in complex with methyl-tetrahydrofolate (P21)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	83.400, 73.100, 89.760
Unit cell angles	90.00, 113.36, 90.00

Refinement procedure

Resolution	30.000 - 1.550
R-factor	0.2194
Rwork	0.209
R-free	0.24330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6sj8
RMSD bond length	0.004
RMSD bond angle	1.276
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.650
High resolution limit [Å]	1.550	1.550
Rmerge	0.071	0.557
Number of reflections	139692	24053
<I/σ(I)>	8.1	2.2
Completeness [%]	97.2
Redundancy	3.1
CC(1/2)	0.997	0.823

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100 mM TRIS, 28% PEG 3350