6SJQ
1.7-A resolution crystal structure of the N-terminal domain of T. brucei BILBO1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 173 |
| Detector technology | CCD |
| Collection date | 2013-07-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.713, 50.793, 37.122 |
| Unit cell angles | 90.00, 94.60, 90.00 |
Refinement procedure
| Resolution | 19.279 - 1.604 |
| R-factor | 0.1699 |
| Rwork | 0.167 |
| R-free | 0.19780 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.165 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.279 | 1.680 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.124 | 1.127 |
| Rpim | 0.044 | 0.410 |
| Number of reflections | 98166 | 1744 |
| <I/σ(I)> | 12.7 | 2.1 |
| Completeness [%] | 99.0 | |
| Redundancy | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M MES NaOH pH 6.5, 30%(w/v) PEG MME 2000 |
