6SJ8
Methyltransferase MtgA from Desulfitobacterium hafniense in complex with tetrahydrofolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.760, 83.850, 86.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.350 |
| R-factor | 0.1215 |
| Rwork | 0.119 |
| R-free | 0.15730 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.472 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.450 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.048 | 0.645 |
| Number of reflections | 120092 | 23073 |
| <I/σ(I)> | 13.8 | 2.1 |
| Completeness [%] | 99.1 | 99.6 |
| Redundancy | 4.1 | |
| CC(1/2) | 0.990 | 0.740 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 100 mM HEPES, 27% PEG 3350 |
