6SJ5

Crystal structure of the uL14-RsfS complex from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-27
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	37.107, 117.601, 121.217
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.203 - 2.267
R-factor	0.230306473507
Rwork	0.229
R-free	0.26359
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o5a 5nd8
RMSD bond length	0.010
RMSD bond angle	1.134
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.290	2.348
High resolution limit [Å]	2.267	2.267
Number of reflections	25254	2494
<I/σ(I)>	14.53	0.9
Completeness [%]	99.0	99.17
Redundancy	4.48	4.39
CC(1/2)	0.999	0.273

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	295	0.2 M Lithium sulfate, 0.1 M MES pH 6.0, 20% PEG 4000