6SIG
Epidermicin antimicrobial protein from Staphylococcus epidermidis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 90.740, 99.440, 69.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.770 - 1.580 |
| R-factor | 0.1575 |
| Rwork | 0.157 |
| R-free | 0.17470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sif |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.693 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.780 | 1.620 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.054 | 0.649 |
| Rmeas | 0.065 | 0.781 |
| Rpim | 0.025 | 0.308 |
| Number of reflections | 42997 | 3140 |
| <I/σ(I)> | 18.3 | |
| Completeness [%] | 99.9 | |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2M Ammonium Sulphate, 0.1M Sodium Acetate pH4.5, 28% PEG 2000 MME |
