6SIE
Crystal structure of the C-lobe of drosophila Arc 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 189.950, 189.950, 40.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| Rwork | 0.231 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6sid |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.315 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmeas | 0.357 | 3.152 |
| Number of reflections | 19015 | 1361 |
| <I/σ(I)> | 8.7 | 0.9 |
| Completeness [%] | 99.8 | 98.3 |
| Redundancy | 14.3 | 13.9 |
| CC(1/2) | 0.992 | 0.433 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.25 M ammonium sulphate, 100 mM Tris pH 8.5, 200 mM lithium sulphate |
