6SID
Crystal structure of the C-lobe of drosophila Arc 1 at atomic resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-14 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.570, 51.100, 82.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.050 |
| Rwork | 0.130 |
| R-free | 0.15100 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.531 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.080 |
| High resolution limit [Å] | 1.050 | 1.050 |
| Rmeas | 0.031 | 0.390 |
| Number of reflections | 34638 | 1229 |
| <I/σ(I)> | 26.1 | 1.6 |
| Completeness [%] | 92.6 | 45.5 |
| Redundancy | 5.9 | 1.6 |
| CC(1/2) | 1.000 | 0.913 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 100 mM MIB buffer (malonic acid, imidazole, boric acid) pH 5, 25 % PEG 1500 |
