6SIB
Crystal structure of the domain-swapped N-lobe dimer of drosophila Arc 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 24.620, 33.420, 84.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.900 |
| Rwork | 0.238 |
| R-free | 0.27100 |
| Structure solution method | AB INITIO PHASING |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.230 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMPLE |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmeas | 0.111 | 1.568 |
| Number of reflections | 5975 | 417 |
| <I/σ(I)> | 9.9 | 1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.8 | 6.1 |
| CC(1/2) | 0.998 | 0.395 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 200 mM ammonium chloride, 100 mM sodium acetate pH 5, 20 % PEG 6000 |
