6SHX
DNA mismatch repair proteins MLH1 and MLH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-05-02 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 93.590, 104.050, 134.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.640 - 2.400 |
| R-factor | 0.214 |
| Rwork | 0.213 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e4w |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.098 | 0.820 |
| Rmeas | 0.106 | 0.910 |
| Rpim | 0.040 | 0.400 |
| Number of reflections | 20049 | 1000 |
| <I/σ(I)> | 11.2 | 1.7 |
| Completeness [%] | 94.0 | 67 |
| Redundancy | 6.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 293 | PEG 4000, imidazole malate, MgCl2 |
