6SG8

Structure of Sosuga virus receptor binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	77
Detector technology	PIXEL
Collection date	2018-04-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 1 21 1
Unit cell lengths	75.700, 84.040, 81.690
Unit cell angles	90.00, 112.85, 90.00

Refinement procedure

Resolution	51.600 - 2.500
R-factor	0.1958
Rwork	0.194
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b2c
RMSD bond length	0.003
RMSD bond angle	0.653
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHENIX
Refinement software	PHENIX (dev_3488)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.600	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.075	0.941
Rmeas	0.081	1.021
Rpim	0.031	0.392
Number of reflections	32731	32699
<I/σ(I)>	13.5	1.5
Completeness [%]	99.7	98.9
Redundancy	6.7	6.5
CC(1/2)	1.000	0.800

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate pH 6.5, 20% PEG 8000