6SF4
Apo form of the ribonucleotide reductase NrdB protein from Leeuwenhoekiella blandensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.8500 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.587, 68.196, 148.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.900 - 1.700 |
R-factor | 0.176 |
Rwork | 0.175 |
R-free | 0.19300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1syy |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.940 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.106 | 2.299 |
Rpim | 0.035 | 0.854 |
Number of reflections | 74093 | 3025 |
<I/σ(I)> | 14.5 | 1 |
Completeness [%] | 97.5 | 76.2 |
Redundancy | 17.5 | 14.8 |
CC(1/2) | 0.999 | 0.538 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Protein at 7.5 mg/ml in buffer containing 50 mM Tris-HCl pH 7.8, 300 mM NaCl, 10% glycerol, 20 mM MgCl2 and 2 mM tris(2-carboxyethyl)phosphine (TCEP). Precipitant 2.4 M sodium malonate dibasic monohydrate pH 7.0. Drops 200+200 nl. |